Ethylenediaminetetraacetic Acid
Ethylenediaminetetraacetic Acid (EDTA) is a chelating agent commonly used in molecular biology buffers. It binds divalent metal ions such as Mg²⁺ and Ca²⁺, which are required cofactors for nucleases. By sequestering these ions, EDTA helps protect DNA and RNA from enzymatic degradation, improves nucleic acid stability, and enhances the reliability of electrophoresis and other downstream molecular biology applications.
Ethylenediaminetetraacetic Acid (EDTA) is a molecular biology–grade, high-purity chelating agent supplied as a 500 g white crystalline powder, widely used in molecular biology and biochemical laboratories to protect nucleic acids. It works by tightly binding divalent metal ions such as Mg²⁺ and Ca²⁺, which are essential cofactors for nucleases, thereby inhibiting DNase and RNase activity and preserving DNA and RNA integrity. EDTA is commonly used for the preparation of standard laboratory buffers including TAE, TE, and EDTA stock solutions, and is compatible with applications such as PCR, agarose gel electrophoresis, restriction enzyme analysis, plasmid DNA preparation, genomic DNA extraction, and RNA handling, ensuring reliable and reproducible results in research and diagnostic workflows.
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